Application
Western blot analysis of L-type Ca++ CP β1b expression in non-transfected 293T: (A), mouse L-type Ca++ CP β1b transfected 293T: (B) and Sol8 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of L-type Ca++ CP β1A expression in Sol8 (A), SK-N-SH (B), L6 (C), C2C12 (D) and L8 (E) whole cell lysates.Application
Western blot analysis of L-type Ca++ CP β1 expression in rat skeletal muscle tissue extract.| Product Name | L-type Ca++ CP β1A Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 211-260 mapping within an internal region of L-type Ca++ CP β1A of human origin |
| Modification Notes | Voltage-dependent Ca2+ channels mediate Ca2+ entry into excitable cells in response to membrane depolarization, and they are involved in a variety of Ca2+-dependent processes, including muscle contraction, hormone or neurotransmitter release and gene expression. Calcium channels are highly diverse, multimeric complexes composed of an å-1 subunit, an intracellular ∫-subunit, a disulfide linked å-2/∂ subunit and a transmembrane ©-subunit. Ca2+ currents are characterized on the basis of their biophysical and pharmacologic properties and include L-, N-, T-, P-, Q-, and R- types. L-type Ca2+ currents initiate muscle contraction, endocrine secretion, and gene transcription, and can be regulated through second-messenger activated protein phosphorylation pathways. L-type calcium channels may form macromolecular signaling complexes with G protein-coupled receptors, thereby enhancing the selectivity of regulating specific targets. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of L-type calcium channel β1 isoform A (also designated isoform 2) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with isoform 1B (also designated isoform 1 or βA) or isoform 1C (also designated isoform 3 or βB) also reactive with additional species, including equine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 211-260 mapping within an internal region of L-type Ca++ CP &beta 1A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 211-260 mapping within an internal region of L-type Ca++ CP β1A of human origin |
| Entrez Gene | 782 |
|---|
Application
Western blot analysis of L-type Ca++ CP β1b expression in non-transfected 293T: (A), mouse L-type Ca++ CP β1b transfected 293T: (B) and Sol8 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human stomach tissue showing cytoplasmic and membrane staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of L-type Ca++ CP β1A expression in Sol8 (A), SK-N-SH (B), L6 (C), C2C12 (D) and L8 (E) whole cell lysates.
Application
Western blot analysis of L-type Ca++ CP β1 expression in rat skeletal muscle tissue extract.| Application Notes | recommended for detection of L-type calcium channel β1 isoform A (also designated isoform 2) of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with isoform 1B (also designated isoform 1 or βA) or isoform 1C (also designated isoform 3 or βB) also reactive with additional species, including equine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
