Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing cytoplasmic staining.Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.Application
Western blot analysis of Sos isoform expression in A-431 (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.| Product Name | Sos 1/2 Antibody (D-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the N-terminus of Sos 1/2 of mouse origin |
| Modification Notes | The superfamily of GTP-binding proteins, of which Ras proteins are prototypes, has been implicated in a broad range of biological activities. Studies have identified a family of guanine nucleotide releasing factors (GRFs) that activate Ras in mammalian cells and an “adapter” protein (Sem 5/GRB2) that appears to mediate the interaction of GRFs with activated receptor molecules (1-3). Ras-GRF p140 promotes nucleotide exchange on ras p21s but not on other members of the Ras gene superfamily. In addition, three mammalian homologs of the Drosophila Ras-GRF, son of sevenless (Sos), have been described (4-6). These include two from mouse, m Sos 1 and m Sos 2, and one from human, h Sos. Vav p95 has been reported to function as a GRF in activation of Ras by the T cell receptor and has been reported to have a domain similar to that of Dbl p115, which is a GRF specific for CDC42Hs. Subsequent to activation, Ras appears to interact with Raf, thereby activating the MAP kinase phosphorylation pathway (7). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Sos 1 p170 and Sos 2 p155 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the N-terminus of Sos 1/2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within the N-terminus of Sos 1/2 of mouse origin |
| Entrez Gene | 6654 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human malignant melanoma tissue showing cytoplasmic staining of tumor cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human lung tumor showing cytoplasmic staining.
Application
Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic staining.
Application
Western blot analysis of Sos isoform expression in A-431 (A), K-562 (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of Sos 1 p170 and Sos 2 p155 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
