Application
Western blot analysis of AQP1 expression in KNRK whole cell lysate (A) and human kidney (B), mouse kidney (C) and rat kidney (D) tissue extracts.Application
Western blot analysis of AQP1 expression in human kidney tissue extract.Application
Immunofluorescence staining of methanol-fixed KNRK cells showing membrane localization.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.Application
Western blot analysis of AQP1 expression in non-transfected control (A) and AQP1 siRNA transfected (B) HeLa cells. Blot probed with AQP1 (H-55): . Actin (I-19): used as specificity and loading control.Application
Western blot analysis of human recombinant AQP1.Application
Western blot analysis of AQP1 expression in mouse kidney tissue extract.| Product Name | AQP1 Antibody (H-55) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 215-269 of aquaporin 1 (AQP1) of human origin |
| Modification Notes | Aquaporins (AQPs) are a large family of integral membrane water transport channel proteins that facilitate the transport of water through the cell membrane. This function is conserved in animals, plants and bacteria. Many isoforms of aquaporin have been identified in mammals, designated AQP0 through AQP10. Aquaporins are widely distributed and it is not uncommon for more than one type of AQP to be present in the same cell. Although most aquaporins are only permeable to water, AQP3, AQP7, AQP9 and one of the two AQP10 transcripts are also permeable to urea and glycerol. AQP2 is the only water channel that is activated by vasopressin to enhance water reabsorption in the kidney collecting duct. Aquaporins are involved in renal water absorption, generation of pulmonary secretions, lacrimation, and the secretion and reabsorption of cerebrospinal fluid and aqueous humor. AQP1 is an integral membrane protein expressed in erythrocytes and renal tubule cells. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of AQP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 215-269 of aquaporin 1 (AQP1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 215-269 of aquaporin 1 (AQP1) of human origin |
| Entrez Gene | 358 |
|---|
Application
Western blot analysis of AQP1 expression in KNRK whole cell lysate (A) and human kidney (B), mouse kidney (C) and rat kidney (D) tissue extracts.
Application
Western blot analysis of AQP1 expression in human kidney tissue extract.
Application
Immunofluorescence staining of methanol-fixed KNRK cells showing membrane localization.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded mouse kidney tissue showing membrane localization.
Application
Western blot analysis of AQP1 expression in non-transfected control (A) and AQP1 siRNA transfected (B) HeLa cells. Blot probed with AQP1 (H-55): . Actin (I-19): used as specificity and loading control.
Application
Western blot analysis of human recombinant AQP1.
Application
Western blot analysis of AQP1 expression in mouse kidney tissue extract.| Application Notes | recommended for detection of AQP1 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
