Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Western blot analysis of Rap 2B expression in non-transfected 293T: (A), human Rap 2B transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of Rap 2C expression in non-transfected: (A) and mouse Rap 2C transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Rap 2C expression in non-transfected 293T: (A), mouse Rap 2C transfected 293T: (B) and A-431 (C) whole cell lysates.Application
Western blot analysis of Rap 2 expression in 293T (A) and T24 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human corpus, uterine tissue showing cytoplasmic staining of glandular cells and cells in endometrial stroma at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of Rap 2 expression in A-431 (A), SW480 (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane staining.| Product Name | Rap 2 Antibody (V-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of Rap 2A of human origin |
| Modification Notes | Ras oncogenes encode GTP-binding proteins that are capable of transforming immortalized cells in culture. Two Ras-related human genes, designated RAP1A and RAP1B, encode 95% homologous proteins (namely Rap 1A and Rap 1B) that share a similar C-terminal Cys-Ali-Ali-Xaa sequence with Ras proteins and are ubiquitously expressed in mammalian tissues. The putative “effector” domain of Ras proteins, whose integrity is required for cell transformation as well as interaction with the putative effector protein GAP, is conserved in both Rap 1 proteins. Rap 1A is thought to interfere with Ras effector function by binding to Ras GAP in a GTP-dependent manner without affecting Rap 1A GTPase activity. Rap 2, another Ras-related protein, shares 60% identity with Rap 1A and exhibits a carboxy terminal CAAX motif and two upstream cysteines similar to those of the H-Ras, K-Ras and N-Ras proteins. In contrast with Rap 1A and Rap 1B, overexpression of Rap 2 does not interfere with the Ras signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Rap 2A, Rap2B and Rap 2C of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of Rap 2A of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of Rap 2A of human origin |
| Entrez Gene | 5911 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Western blot analysis of Rap 2B expression in non-transfected 293T: (A), human Rap 2B transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of Rap 2C expression in non-transfected: (A) and mouse Rap 2C transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Rap 2C expression in non-transfected 293T: (A), mouse Rap 2C transfected 293T: (B) and A-431 (C) whole cell lysates.
Application
Western blot analysis of Rap 2 expression in 293T (A) and T24 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human corpus, uterine tissue showing cytoplasmic staining of glandular cells and cells in endometrial stroma at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of Rap 2 expression in A-431 (A), SW480 (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-431 cells showing membrane staining.| Application Notes | recommended for detection of Rap 2A, Rap2B and Rap 2C of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
