Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of ARF1 expression in non-transfected: (A) and human ARF1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ARF expression in mouse placenta tissue extract.Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of ARF expression in HeLa (A), Caki-1 (B) and KNRK (C) whole cell lysates.| Product Name | ARF Antibody (H-50) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 128-177 mapping near the C-terminus of ADP-ribosylation factor 1 of human origin |
| Modification Notes | The ADP-ribosylation factor (ARF) protein family are structurally and functionally conserved members of the Ras superfamily of regulatory GTP-binding proteins (1–3). ARFs influence vesicle trafficking and signal transduction in eukaryotic cells (1–3). ARF-dependent regulatory mechanisms include the coordination of spectrin interactions with golgi membranes and the association of actin to the golgi via rho family-dependent G-protein localization (Rac, CDC42) and WASP/Arp2/3 complexes (3–7). Additionally, ARFs play a central role in maintenance of organelle integrity, assembly of coat proteins, and activation of phospho-lipase D (5–7). The ARF proteins are categorized as class I (ARF1, ARF2, and ARF3), class II (ARF4 and ARF5) and class III (ARF6); members of each class share a common gene organization (8,9). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of ARF family proteins of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 128-177 mapping near the C-terminus of ADP-ribosylation factor 1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 128-177 mapping near the C-terminus of ADP-ribosylation factor 1 of human origin |
| Entrez Gene | 375 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing membrane localization.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of ARF1 expression in non-transfected: (A) and human ARF1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ARF expression in mouse placenta tissue extract.
Application
Intracellular FCM analysis of fixed and permeabilized HeLa cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of ARF expression in HeLa (A), Caki-1 (B) and KNRK (C) whole cell lysates.| Application Notes | recommended for detection of ARF family proteins of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
