Application
Western blot analysis of NEDD4 expression in KNRK whole cell lysate.Application
Immunofluorescence staining of methanol-fixed Caki-1 cells showing cytoplasmic localization.| Product Name | NEDD4-1 Antibody (H-135) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 560-694 of NEDD4-1 of human origin |
| Modification Notes | NEDD4 is an E3 ubiquitin-protein ligase that contains several family members including NEDD4-1 and NEDD4-2, both of which are HECT-type E3 ligases with similar structure and function. Located primarily in muscle fibers, NEDD4 is a ubiquitin acceptor that transports ubiquitin from E2 ligase enzymes to various substrates throughout the body, helping to regulate degradation of plasma membranes. Thought to play a role in the budding of many retroviruses, NEDD4 interacts with the viral protein motif P-P-P-Y, an interaction that is important for viral maturation. NEDD4 contains three WW domains to which many ubiquitin-receiving proteins, including NEDD4 family interacting protein-1 (NDFIP1), bind and interact. NDFIP1 is strongly expressed in surviving neurons around a site of injury, suggesting that ubiquitination may be a possible survival strategy and NDFIP1 may act as a neuroprotective protein. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of NEDD4-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 560-694 of NEDD4-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 560-694 of NEDD4-1 of human origin |
| Entrez Gene | 4734 |
|---|
Application
Western blot analysis of NEDD4 expression in KNRK whole cell lysate.
Application
Immunofluorescence staining of methanol-fixed Caki-1 cells showing cytoplasmic localization.| Application Notes | recommended for detection of NEDD4-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
