Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic and membrane staining of glandular cells.Application
Western blot analysis of JAM-A expression in human PBL whole cell lysate.Application
Western blot analysis of JAM-A expression in non-transfected: (A) and mouse JAM-A transfected: (B) 293T whole cell lysates.Application
Western blot analysis of JAM-A expression in human platelet whole cell lysate.| Product Name | JAM-A Antibody (H-80) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 220-299 mapping at the C-terminus of JJAM-A of human origin |
| Modification Notes | Junctional adhesion molecule (JAM) is a member of the immunoglobulin superfamily expressed in tight junctions of epithelial cells and endothelial cells. It is implicated in transendothelial migration of leukocytes. JAM is constitutively expressed on circulating monocytes, neutrophils, lymphocyte subsets and platelets. The JAM family consists of JAM-A, JAM-B and JAM-C, alternatively designated JAM-1, JAM-2 and JAM-3, respectively. JAM-A localizes with F-Actin at the cell-cell contacts and at the membrane ruffles. It is involved in cell to cell adhesion through homophilic interactions and plays a role in the organization of tight junctions and modulation of leukocyte extravasation. JAM-B interacts with discrete subsets of PBLs, suggesting that it may play a role in lymphocyte trafficking. JAM-B and JAM-C proteins are binding partners; JAM-C may be a functional JAM-B receptor. Specifically, JAM-B adheres to T cells through heterotypic interactions with JAM-C. The JAM-B/JAM-C interaction may play a role in T, NK and dendritic cellular inflammation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of JAM-A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 220-299 mapping at the C-terminus of JJAM-A of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 220-299 mapping at the C-terminus of JJAM-A of human origin |
| Entrez Gene | 50848 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon tissue showing cytoplasmic and membrane staining of glandular cells.
Application
Western blot analysis of JAM-A expression in human PBL whole cell lysate.
Application
Western blot analysis of JAM-A expression in non-transfected: (A) and mouse JAM-A transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of JAM-A expression in human platelet whole cell lysate.| Application Notes | recommended for detection of JAM-A of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
