Application
Western blot analysis of SARA expression in non-transfected: (A) and human SARA transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.Application
Western blot analysis of SARA expression in HeLa whole cell lysate.| Product Name | SARA Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 of SARA of human origin |
| Modification Notes | SARA (NSP, SARA, MADHIP, SMADIP, ZFYVE9, zinc finger FYVE domain containing 9) is a double zinc finger (FYVE domain) protein that influences the recruitment of Smad proteins to the TGF-beta receptor and ensures appropriate subcellular localization of the activated receptor-bound complex. The FYVE domain in SARA directs localization to early endosomal compartments where it can interact with TGF-beta receptors and Smads. Promyelocytic leukemia (PML) tumour suppressor physically interacts with Smad2/3 and SARA and promotes association and accumulation of SARA and TGF-beta receptor in early endosome. SARA can enhance recruitment of protein phosphatase 1 catalytic subunit (PP1c) to Smad7-GADD34 complex by controlling the specific subcellular localization of PP1c. Dephosphorylation of TGF-beta receptor by Smad7 is an effective mechanism for governing negative feedback in TGF-beta signaling. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SARA of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 of SARA of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 of SARA of human origin |
| Entrez Gene | 9372 |
|---|
Application
Western blot analysis of SARA expression in non-transfected: (A) and human SARA transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.
Application
Western blot analysis of SARA expression in HeLa whole cell lysate.| Application Notes | recommended for detection of SARA of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
