Application
Western blot analysis of FTα expression in NIH/3T3 whole cell lysate.Application
Western blot analysis of FTα expression in non-transfected 293: (A), human FTα transfected 293: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of FTα expression in non-transfected: (A) and human FTα transfected: (B) 293 whole cell lysates.Application
Western blot analysis of FTα expression in mouse placenta tissue extract (A) and 3T3-L1 (B) and NIH/3T3 (C) whole cell lysates.Application
Western blot analysis of FTα expression in mouse heart tissue extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.| Product Name | FTα Antibody (FL-379) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Mammalian protein farnesyl transferases are heterodimeric proteins containing two nonidentical a and b subunits that attach farnesyl residues to a cysteine at the fourth position from the COOH terminus of several proteins, including nuclear lamins and p21Ras proteins. The natural substrates contain the Cys-A-A-Xaa recognition sequence, where the A residues are aliphatic and Xaa represents methionine, serine, glutamine or cysteine. The purified farnesyl transferase is an ab heterodimer. The b subunit binds the peptide substrate while the a subunit is suspected to participate in formation of a stable complex with the substrate farnesyl pyrophosphate. The a subunit is shared with a second prenyltransferase, geranylgeranyl transferase, that attaches 20 carbon geranylgeranyl to Ras-related proteins that terminate in a Cys-A-A-Xaa recognition site in which Xaa is leucine. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA |
| recommended for detection of FT&alpha of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-379 representing the full length 49 kDa &alpha subunit common to farnesyl transferase and geranylgeranyl transferase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-379 representing the full length 49 kDa α subunit common to farnesyl transferase and geranylgeranyl transferase of human origin |
| Entrez Gene | 2339 |
|---|
Application
Western blot analysis of FTα expression in NIH/3T3 whole cell lysate.
Application
Western blot analysis of FTα expression in non-transfected 293: (A), human FTα transfected 293: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of FTα expression in non-transfected: (A) and human FTα transfected: (B) 293 whole cell lysates.
Application
Western blot analysis of FTα expression in mouse placenta tissue extract (A) and 3T3-L1 (B) and NIH/3T3 (C) whole cell lysates.
Application
Western blot analysis of FTα expression in mouse heart tissue extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing perinuclear localization.| Application Notes | recommended for detection of FT&alpha of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
