Application
Western blot analysis of HSP 105 expression in untreated (A) and Lactacystin treated (B) C6 whole cell lysates. Note upregulation of HSP 105 expression in lane B.Application
Western blot analysis of HSP 105 expression in non-transfected 293T: (A), human HSP 105 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HSP 105 expression in non-transfected 293T: (A), human HSP 105 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of HSP 105 expression in HeLa whole cell lysate.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining.Application
HSP 105 (N-187): .Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization .| Product Name | HSP 105 Antibody (N-187) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 187-512 centrally located within the 858 residue of HSP 105 of mouse origin |
| Modification Notes | The heat shock proteins (HSPs) comprise a group of highly conserved, abundantly expressed proteins with diverse functions, including the assembly and sequestering of multiprotein complexes, transportation of nascent poly-peptide chains across cellular membranes and regulation of protein folding. Heat shock proteins (also known as molecular chaperones) fall into six general families: HSP 90, HSP 70, HSP 60, the low molecular weight HSPs, the immunophilins and the HSP 110 family. The HSP 110 family (also known as the HSP 105 family) is composed of HSP 105, Apg-1 and Apg-2. HSP 105 is a testis-specific and HSP 90-related protein. Research indicates that HSP 105 is specifically localized in the germ cells and may translocate into the nucleus after heat shock. It is suggested that HSP 105 may contribute to the stabilization of p53 proteins in the cytoplasm of the germ cells, preventing the potential induction of apoptosis by p53. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of HSP 105 and, to a lesser extent, Apg-1 and Apg-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 187-512 centrally located within the 858 residue of HSP 105 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 187-512 centrally located within the 858 residue of HSP 105 of mouse origin |
| Entrez Gene | 10808 |
|---|
Application
Western blot analysis of HSP 105 expression in untreated (A) and Lactacystin treated (B) C6 whole cell lysates. Note upregulation of HSP 105 expression in lane B.
Application
Western blot analysis of HSP 105 expression in non-transfected 293T: (A), human HSP 105 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HSP 105 expression in non-transfected 293T: (A), human HSP 105 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of HSP 105 expression in HeLa whole cell lysate.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing nuclear staining.
Application
HSP 105 (N-187): .Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic localization .| Application Notes | recommended for detection of HSP 105 and, to a lesser extent, Apg-1 and Apg-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
