Application
Western blot analysis of EGFR expression in EGF treated A-431 (A), HeLa (B) and C3H/10T1/2 (C) whole cell lysates.Application
Western blot analysis of EGFR expression in BT-20 non-reducing buffer, not boiled (A) and HeLa non-reducing buffer, not boiled (B) whole cell lysates.Application
Western blot analysis of EGFR expression in A-431 whole cell lysate.Application
Western blot analysis of EGFR expression in C3H/10T1/2 whole cell lysate.Application
Western blot analysis of EGFR expression in non-transfected control (A) and EGFR siRNA transfected (B) A-431 cells. Blot probed with EGFR (1005): .Application
Western blot analysis of EGFR phosphorylation in untreated (A) and EGF-stimulated (B) A-431 cells. EGFR levels in the same untreated (C) and treated (D) cultures were determined by using EGFR (1005): .Application
Antibodies tested include p-EGFR (Tyr 1173)-R: (A,B) and EGFR (1005): (C,D).Application
Western blot analysis of EGFR expression in A-431 human carcinoma cells.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane staining.| Product Name | EGFR Antibody (1005) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of EGFR of human origin |
| Modification Notes | The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Exons in the EGFR gene product are frequently either deleted or duplicated to produce deletion mutants (DM) or tandem duplication mutants (TDM), respectively, which are detected at various molecular weights. EGFR binds several ligands, including epidermal growth factor (EGF), transforming growth factor å (TGFå), Amphiregulin and heparin binding-EGF (HB-EGF). Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of EGF receptor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, may detect highly overexpressed ErbB-2 also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of EGFR of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of EGFR of human origin |
| Entrez Gene | 1956 |
|---|
Application
Western blot analysis of EGFR expression in EGF treated A-431 (A), HeLa (B) and C3H/10T1/2 (C) whole cell lysates.
Application
Western blot analysis of EGFR expression in BT-20 non-reducing buffer, not boiled (A) and HeLa non-reducing buffer, not boiled (B) whole cell lysates.
Application
Western blot analysis of EGFR expression in A-431 whole cell lysate.
Application
Western blot analysis of EGFR expression in C3H/10T1/2 whole cell lysate.
Application
Western blot analysis of EGFR expression in non-transfected control (A) and EGFR siRNA transfected (B) A-431 cells. Blot probed with EGFR (1005): .
Application
Western blot analysis of EGFR phosphorylation in untreated (A) and EGF-stimulated (B) A-431 cells. EGFR levels in the same untreated (C) and treated (D) cultures were determined by using EGFR (1005): .
Application
Antibodies tested include p-EGFR (Tyr 1173)-R: (A,B) and EGFR (1005): (C,D).
Application
Western blot analysis of EGFR expression in A-431 human carcinoma cells.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human breast carcinoma tissue showing membrane staining.| Application Notes | recommended for detection of EGF receptor of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, may detect highly overexpressed ErbB-2 also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
