Application
Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal mouse skin showing cytoplasmic and membrane staining.Application
Western blot analysis of PDGFR-α expression in NIH/3T3 whole cell lysates (A,B).| Product Name | PDGFR-α Antibody (C-20) AC |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the C-terminus of PDGFR-α of human origin |
| Modification Notes | Platelet-derived growth factor (PDGF) is a mitogen for mesenchyme- and glia-derived cells. PDGF consists of two chains, A and B, which dimerize to form functionally distinct isoforms PGDF-AA, PDGF-AB and PDGF-BB. These three isoforms bind with different affinities to two receptor types, PDGFR-å and -∫, which are endowed with protein tyrosine kinase domains. PDGFR-å can bind to both A and B subunits of PDGF, while PDGFR-∫ can only bind the B subunit. Ligand binding promotes either homo- or heterodimerization of the PDGF receptors in a specific manner. PDGF-AA induces the dimerization of two a receptors, PDGF-AB induces dimerization of åå and å∫, and PDGF-BB induces the formation of three types of dimers: åå, å∫ and ∫∫. The genes encoding PDGFR-å and -∫ map to human chromosome 4q12 and 5q33.1, respectively. Translocation of the PDGFR-b gene with the TEL gene is linked with chronic myelomonocytic leukemia (CMML), a myelodysplastic syndrome, and demonstrates the oncogenic potential of the PDGF receptors. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCFCELISA |
| recommended for detection of PDGF receptor type α of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the C-terminus of PDGFR-&alpha of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the C-terminus of PDGFR-α of human origin |
| Entrez Gene | 5156 |
|---|
Application
Intracellular FCM analysis of fixed and permeabilized NIH/3T3 cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded normal mouse skin showing cytoplasmic and membrane staining.
Application
Western blot analysis of PDGFR-α expression in NIH/3T3 whole cell lysates (A,B).| Application Notes | recommended for detection of PDGF receptor type α of mouse, rat and human origin by WB, IP, IF, IHC(P), FCM and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
