Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic localization.| Product Name | Tyrosinase Antibody (H-109) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 421-529 mapping at the C-terminus of Tyrosinase of human origin |
| Modification Notes | Tyrosinase (TYR), a type I membrane protein and copper-containing enzyme, is involved in the production of melanin, the primary pigment found in vertebrates. Melanin biogenesis requires the enzymatic activity of TYR, which catalyzes the critical and rate-limiting step of tyrosine hydroxylation in the biosynthesis of melanin. Defects effecting TYR activity result in various forms of albinism. The TYR-related proteins, TRP1 and TRP2, are also specifically expressed in melanocytes, and they likewise contribute to the synthesis of melanin within the melanosomes. The TRPs, including TYR, all share a similar transmembrane region, contain two metal-binding regions and a cysteine-rich epidermal growth factor motif, and are localized in the melanosomal membrane. These proteins, however, have distinct catalytic activity, and they individually contribute to the biosynthesis of melanin biopolymers. The TRPs are believed to exists as a multi-enzyme complex, as these proteins form aggregates together, and the expression of TRP1 also helps stabilize TYR in melanocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Tyrosinase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 421-529 mapping at the C-terminus of Tyrosinase of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 421-529 mapping at the C-terminus of Tyrosinase of human origin |
| Entrez Gene | 7299 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.
Application
Immunofluorescence staining of normal mouse intestine frozen section showing cytoplasmic localization.| Application Notes | recommended for detection of Tyrosinase of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
