Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic localization.Application
Western blot analysis of PLCβ2 expression in RAW 264.7 whole cell lysate.| Product Name | PLC β2 Antibody (Q-15) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping near the C-terminus of PLC β2 of human origin |
| Modification Notes | Phosphoinositide-specific phospholipase C (PLC) plays a crucial role in the initiation of receptor mediated signal transduction through the generation of the two second messengers, inositol 1,4,5-triphosphate and diacylglycerol from phosphatidylinositol 4,5-bisphosphate. There are many mammalian PLC isozymes, including PLC ∫1, PLC ∫2, PLC ∫3, PLC ∫4, PLC©1, PLC©2, PLC ∂1, PLC ∂2 and PLCe). PLC ∫s are the only PLC isforms that are regulated by G protein subunits and are activated by a heterotrimeric GTP-binding protein linked to various cell surface receptors. Two alternatively spliced forms (1181 and 1166 amino acids) of PLC ∫2 are generated in hematopoietic cells that differ in the carboxyl terminal sequence implicated in interaction of PLC ∫ enzymes with Gåq.The pleckstrin homology domain of PLC ∫2 is required for its targeting to the membrane and for substrate hydrolysis and its linker region exerts an inhibitory effect on basal PLC ∫2 activity. PLC ∫2 plays a major role in platelet activation and is mainly expressed in the periphery of the islet and acinar cells in rat pancreas. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of PLC β2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of PLC &beta 2 of human origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of PLC β2 of human origin |
| Entrez Gene | 5330 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human pancreas tissue showing cytoplasmic staining of exocrine pancreas and islet cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells showing cytoplasmic localization.
Application
Western blot analysis of PLCβ2 expression in RAW 264.7 whole cell lysate.| Application Notes | recommended for detection of PLC β2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
