Application
Western blot analysis of Ly-GDI expression in non-transfected 293T: (A), mouse Ly-GDI transfected 293T: (B) and U-937 (C) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of bone marrow poietic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Immunofluorescence staining of methanol-fixed BJAB cells showing cytoplasmic localization.Application
Western blot analysis of Ly-GDI expression in BJAB whole cell lysates.| Product Name | Ly-GDI Antibody (FL-201) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-201 of Ly-GDI of human origin |
| Modification Notes | The Ras superfamily of small GTP-binding proteins are critical mediators of diverse cell signaling pathways, including those leading to proliferation, cytoskeletal organization and secretion. The counter-conversion of the active GTP-bound form of these proteins to their inactive GDP-bound form is influenced by two types of regulatory proteins: those that alter the intrinsic GTPase activity of the GTP-binding proteins and those that alter the rate of GDP/GTP exchange. Guanine nucleotide-releasing factors (GRFs) increase the GDP dissociation rate, while GDP-dissociation inhibitors (GDIs) decrease the dissociation rate. The Rho GDI subfamily is composed of Rho GDIå, Ly-GDI (also known as Rho GDI∫ and previously known as GDI/D4) and Rho GDI©. The Rho GDI proteins interact with and have varying affinities for several Ras-like GTP binding proteins, including Rho A, Rho B, Rac and Cdc42. Ly-GDI is expressed only in hematopoietic cells, predominantly in B and T lymphocyte cell lines. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of Ly-GDI and, to a lesser extent, Rho GDIα and Rho GDIγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-201 of Ly-GDI of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-201 of Ly-GDI of human origin |
| Entrez Gene | 397 |
|---|
Application
Western blot analysis of Ly-GDI expression in non-transfected 293T: (A), mouse Ly-GDI transfected 293T: (B) and U-937 (C) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bone marrow tissue showing cytoplasmic staining of bone marrow poietic cells (low and high magnification). Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Immunofluorescence staining of methanol-fixed BJAB cells showing cytoplasmic localization.
Application
Western blot analysis of Ly-GDI expression in BJAB whole cell lysates.| Application Notes | recommended for detection of Ly-GDI and, to a lesser extent, Rho GDIα and Rho GDIγ of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
