Application
Western blot analysis of NOS2 expression in Raw 264.7 (A), LPS treated Raw 264.7 (B) and LPS and Tanshinone IIA treated Raw 264.7 (C) whole cell lysates.Application
Western blot analysis of whole cell lysates prepared from untreated (A) and phorbol ester and LPS-treated (B) cultures of RAW 264.7.Application
Antibodies tested include NOS2 (N-20): and NOS2 (M-19): (C,D).Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.| Product Name | NOS2 Antibody (M-19) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | Nitric oxide (NO) has a broad range of biological activities and has been implicated in signaling pathways in phylogenetically diverse species. Nitric oxide synthases (NOSs), the enzymes responsible for synthesis of NO, contain an N-terminal oxygenase domain and a C-terminal reductase domain. NOS activity requires homodimerization as well as three cosubstrates (L-arginine, NADPH and O2) and five cofactors or prosthetic groups (FAD, FMN, calmodulin, tetrahydrobiopterin and heme). Several distinct NOS isoforms have been described and been shown to represent the products of three distinct genes. These include two constitutive Ca2+/CaM-dependent forms of NOS, including NOS1 (also designated ncNOS) whose activity was first identified in neurons, and NOS3 (also designated ecNOS), first identified in endothelial cells. The inducible form of NOS, NOS2 (also designated iNOS), is Ca2+-independent and is expressed in a broad range of cell types. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,ELISA |
| recommended for detection of NOS2 (iNOS) of mouse and rat origin by WB, IP, IF and ELISA | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping near the C-terminus of NOS2 of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping near the C-terminus of NOS2 of mouse origin |
| Entrez Gene | 18126 |
|---|
Application
Western blot analysis of NOS2 expression in Raw 264.7 (A), LPS treated Raw 264.7 (B) and LPS and Tanshinone IIA treated Raw 264.7 (C) whole cell lysates.
Application
Western blot analysis of whole cell lysates prepared from untreated (A) and phorbol ester and LPS-treated (B) cultures of RAW 264.7.
Application
Antibodies tested include NOS2 (N-20): and NOS2 (M-19): (C,D).
Application
Immunofluorescence staining of methanol-fixed RAW 264.7 cells stimulated with LPS/IFN-γ showing cytoplasmic staining.| Application Notes | recommended for detection of NOS2 (iNOS) of mouse and rat origin by WB, IP, IF and ELISA |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
