Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.Application
Western blot analysis of Nrf1 expression in non-transfected: (A) and human Nrf1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Nrf1 expression in non-transfected: (A) and human Nrf1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Nrf1 expression in non-transfected: (A) and mouse Nrf1 transfected: (B) 293T whole cell lysates.| Product Name | Nrf1 Antibody (H-285) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 191-475 mapping near the N-terminus of Nrf1 of human origin |
| Modification Notes | The NF-E2 DNA binding protein is composed of two subunits, p45 and MafK, and it regulates expression of globin genes in developing erythroid cells through interaction with Maf recognition elements (MAREs). A family of NF-E2-related proteins, which are collectively known as the cap ‘n’ collar (CNC) family and include Nrf1 (also designated TCF11), Nrf2 and Nrf3, are bZIP transcription factors that heterodimerize with Maf proteins to bind MARE sequences. The Nrf proteins also bind the antioxidant response element (ARE) and are implicated in the regulation of detoxification enzymes and the oxidative stress response. They do so by heterodimerizing with Jun family members (c-Jun, Jun B and Jun D) to activate gene expression, specifically the detoxifying enzyme NQO1. Nrf2 is widely expressed and is thought to translocate to the nucleus after treatment with xenobiotics and antioxidants, which stimulate its release from a repressor protein Keap1. The gene encoding human Nrf3 maps to chromosome 7p15.2. Nrf3 is highly expressed in placenta, B cells and monocytes. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Nrf1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 191-475 mapping near the N-terminus of Nrf1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 191-475 mapping near the N-terminus of Nrf1 of human origin |
| Entrez Gene | 4779 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear and cytoplasmic localization.
Application
Western blot analysis of Nrf1 expression in non-transfected: (A) and human Nrf1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Nrf1 expression in non-transfected: (A) and human Nrf1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Nrf1 expression in non-transfected: (A) and mouse Nrf1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of Nrf1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
