Application
Western blot analysis of BAF155 expression in non-transfected 293: (A), human BAF155 transfected 293: (B) and Jurkat (C) whole cell lysates.Application
Western blot analysis of BAF155 expression in non-transfected 293T: (A), human BAF155 transfected 293T: (B) and K-562 (C) whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.Application
Western blot analysis of BAF155 expression in HeLa (A) and K-562 (B) whole cell lysates.| Product Name | BAF155 Antibody (H-76) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 998-1073 of BAF155 of human origin |
| Modification Notes | The SWI/SNF complex is involved in the activation of transcription via the remodeling of nucleosome structure in an ATP-dependent manner. Brm (also designated SNF1 or SNF2å) and Brg-1 (also designated SNF2 or SNF2∫) are the ATPase subunits of the mammalian SWI/SNF complex. Brm, Brg-1, Ini1 (integrase interactor 1, also designated SNF5), BAF155 (also designated SRG3) and BAF170 are thought to comprise the functional core of the SWI/SNF complex. Addition of Ini1, BAF155 and BAF170 to Brg-1 appears to increase remodeling activity. Other complex subunits are thought to play regulatory roles. hSNF2L and hSNF2H both appear to be homologs of Drosophila ISWI, a Brm related ATPase that is present in chromatin remodeling complexes other than SWI/SNF, including the NURF (nucleosome remodeling factor). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of BAF155 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 998-1073 of BAF155 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 998-1073 of BAF155 of human origin |
| Entrez Gene | 6599 |
|---|
Application
Western blot analysis of BAF155 expression in non-transfected 293: (A), human BAF155 transfected 293: (B) and Jurkat (C) whole cell lysates.
Application
Western blot analysis of BAF155 expression in non-transfected 293T: (A), human BAF155 transfected 293T: (B) and K-562 (C) whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear staining.
Application
Western blot analysis of BAF155 expression in HeLa (A) and K-562 (B) whole cell lysates.| Application Notes | recommended for detection of BAF155 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine and canine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
