Application
Western blot analysis of p19 ARF expression in C3H/10T1/2 (A), NIH/3T3 (B), 3T3-L1 (C), MH-S (D), AMJ2-C8 (E) and I-11.15 (F) whole cell lysates.| Product Name | p19 ARF Antibody (M-60) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 6-65 mapping near the N-terminus of p19 ARF of mouse origin |
| Modification Notes | The progression of cells through the cell cycle is regulated by a family of proteins designated cyclin-dependent kinases (Cdks). Sequential activation of individual members of this family and their consequent phosphorylation of critical substrates, promote orderly progression through the cell cycle. The protein p16INK4A, identified as a negative regulator of the cell cycle, has been shown to bind to and inhibit the activity of the Cdk4/cyclin D complex. p19 ARF, which is unrelated to p16, arises from transcription of an alternative reading frame of the p16 gene. Like p16, p19 ARF has been shown to induce cell cycle arrest. Mice lacking p19 ARF but expressing functional p16 have been shown to develop tumors early in life. Further studies have indicated that p19 ARF may be disrupted in a large percentage of human T cell acute lymphoblastic leukemias. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of p19 ARF of mouse origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 6-65 mapping near the N-terminus of p19 ARF of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 6-65 mapping near the N-terminus of p19 ARF of mouse origin |
| Entrez Gene | 12578 |
|---|
Application
Western blot analysis of p19 ARF expression in C3H/10T1/2 (A), NIH/3T3 (B), 3T3-L1 (C), MH-S (D), AMJ2-C8 (E) and I-11.15 (F) whole cell lysates.| Application Notes | recommended for detection of p19 ARF of mouse origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
