Application
Western blot analysis of MAGE expression in Hs 294T whole cell lysate.Application
Western blot analysis of MAGE expression in A-375 whole cell lysate.Application
Western blot analysis of MAGE-A1 expression in non-transfected: (A) and human MAGE-A1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of MAGE-A1 expression in non-transfected: (A) and human MAGE-A1 transfected: (B) 293 whole cell lysates.Application
Immunofluorescence staining of methanol-fixed A-375 cells showing cytoplasmic localization.| Product Name | MAGE Antibody (FL-309) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-309 representing full length MAGE-A1 of human origin |
| Modification Notes | The melanoma-associated antigen (MAGE) family consists of a number of antigens recognized by cytotoxic T lymphocytes. The MAGE genes were initially isolated from different kinds of tumors, and based on their virtually exclusive tumor-specific expression in adult tissues, they have been used as targets for cancer immunotherapy. MAGE genes encode for tumor-rejection antigens and are expressed in tumors of different histologic types, but not in normal tissues, with the exception of testis and placenta. Although a large number of MAGE genes have now been identified and extensively studied in tumors of various origin, their function in normal cells remains unknown. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of all MAGE family members of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-309 representing full length MAGE-A1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-309 representing full length MAGE-A1 of human origin |
| Entrez Gene | 4100 |
|---|
Application
Western blot analysis of MAGE expression in Hs 294T whole cell lysate.
Application
Western blot analysis of MAGE expression in A-375 whole cell lysate.
Application
Western blot analysis of MAGE-A1 expression in non-transfected: (A) and human MAGE-A1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of MAGE-A1 expression in non-transfected: (A) and human MAGE-A1 transfected: (B) 293 whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed A-375 cells showing cytoplasmic localization.| Application Notes | recommended for detection of all MAGE family members of human and, to a lesser extent, mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
