Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells and interstitial cells.Application
Western blot analysis of BRCA1 expression in mouse embryo tissue extract.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of human recombinant BRCA1 fusion protein.| Product Name | BRCA1 Antibody (D-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Modification Notes | In 1990, a breast cancer susceptibility gene, designated BRCA1, was localized to chromosome 17q. Mutations within this gene are believed to account for approximately 45% of families with high incidence of breast cancer and at least 80% of families with increased incidence of both early-onset breast cancer and ovarian cancer. A second breast cancer susceptibility gene, BRCA2, located on chromosome 13q12-13, also confers a high incidence of breast cancer but, unlike BRCA1, does not confer a substantially elevated risk of ovarian cancer. The BRCA1 gene is expressed in numerous tissues, including breast and ovary, and encodes a predicted protein of 1863 amino acids. This protein contains a zinc finger domain in its amino terminal region, but is otherwise unrelated to any previously described proteins. Like many other genes involved in familial cancer, BRCA1 appears to encode a tumor suppressor, a protein that acts as a negative regulator of tumor growth. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB,IP,IF,IHC,ELISA |
| recommended for detection of BRCA1 of human and, to a lesser extent, mouse origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with BRCA2 or EGFR also reactive with additional species, including equine, canine and bovine | |
| Species Reactivity | Human,Mouse,Rat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of BRCA1 of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of BRCA1 of human origin |
| Entrez Gene | 672 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human bronchus tissue showing nuclear and cytoplasmic staining of respiratory epithelial cells and interstitial cells.
Application
Western blot analysis of BRCA1 expression in mouse embryo tissue extract.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of human recombinant BRCA1 fusion protein.| Application Notes | recommended for detection of BRCA1 of human and, to a lesser extent, mouse origin by WB, IP, IF, IHC(P) and ELISA non cross-reactive with BRCA2 or EGFR also reactive with additional species, including equine, canine and bovine |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
