Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic and nuclear staining of subset of alveolar cells.Application
Western blot analysis of SP-B expression in non-transfected: (A) and human SP-B transfected: (B) 293T whole cell lysates.| Product Name | SP-B Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of pulmonary surfactant-associated protein B (SP-B or PSAP B) of human origin |
| Modification Notes | Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C, SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of SP-B of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of pulmonary surfactant-associated protein B (SP-B or PSAP B) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of pulmonary surfactant-associated protein B (SP-B or PSAP B) of human origin |
| Entrez Gene | 6439 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic and nuclear staining of subset of alveolar cells.
Application
Western blot analysis of SP-B expression in non-transfected: (A) and human SP-B transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of SP-B of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
