Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of pneumocytes and macrophages.Application
Western blot analysis of SP-C expression in Mv 1 Lu (A), AMJ2-C8 (B), AMJ2-C11 (C), MH-S (D) and P 23 (E) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of alveolar cells and macrophages at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of SP-C expression in rat lung extract.| Product Name | SP-C Antibody (FL-197) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-197 representing full length pulmonary surfactant protein C (SP-C) of human origin |
| Modification Notes | Pulmonary surfactant is primarily responsible for lowering the surface tension at the air-liquid interface in the alveoli, a process that is essential for normal respiration. Pulmonary surfactant is a mixture of phospholipids and proteins, including four distinct surfactant-associated proteins (SPs), SP-A, SP-B, SP-C and SP-D. SP-B and SP-C are predominantly hydrophobic proteins that associate with lipids to promote the absorption of surfactant phospholipids and to reduce the surface tension in the alveoli. SP-A and SP-D are large multimeric proteins belonging to the family of calcium-dependent lectins, designated collectins, which contribute to the innate immune system. Both SP-A and SP-D have been shown to protect against microbial challenge through binding to the lipid components of the bacterial cell wall and facilitating the rapid removal of microbials. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of SP-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~5000 IP:1:20~1:100 |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-197 representing full length pulmonary surfactant protein C (SP-C) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-197 representing full length pulmonary surfactant protein C (SP-C) of human origin |
| Entrez Gene | 6440 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of pneumocytes and macrophages.
Application
Western blot analysis of SP-C expression in Mv 1 Lu (A), AMJ2-C8 (B), AMJ2-C11 (C), MH-S (D) and P 23 (E) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human lung tissue showing cytoplasmic staining of alveolar cells and macrophages at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of SP-C expression in rat lung extract.| Application Notes | recommended for detection of SP-C of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: WB:1:100~1:200 IHC:1:10~1:50 IF:1:20~1:100 ELISA:1:1000~5000 IP:1:20~1:100 |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
