Application
Western blot analysis of FAS-L expression in K-562 (A) and HL-60 (B) whole cell lysates immunoprecipitated with FAS-L (4H9): and detected with FAS-L (N-20): .Application
Western blot analysis of FAS-L expression in K-562 (A) and HL-60 (B) whole cell lysates immunoprecipitated with FAS-L (NOK-1): and detected with FAS-L (N-20): .Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue at high magnification showing membrane and cytoplasmic localization.| Product Name | FAS-L Antibody (N-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of FAS-L of rat origin |
| Modification Notes | Cytotoxic T lymphocyte (CTL)-mediated cytotoxicity constitutes an important component of specific effector mechanisms in immunosurveillance against virus-infected or transformed cells. Two mechanisms appear to account for this activity, one of which is the perforin-based process. Independently, a FAS-based mechanism involves the transducing molecule FAS (also designated Apo-1) and its ligand (FAS-L). The human FAS protein is a cell surface glycoprotein that belongs to a family of receptors that includes CD40, nerve growth factor receptors and tumor necrosis factor receptors. The FAS antigen is expressed on a broad range of lymphoid cell lines, certain of which undergo apoptosis in response to treatment with antibody to FAS. These findings strongly imply that targeted cell death is potentially mediated by the intercellular interactions of FAS with its ligand or effectors, and that FAS may be critically involved in CTL-mediated cytotoxicity. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIFIHCELISA |
| recommended for detection of FAS-L of mouse, rat and human origin by WB, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of FAS-L of rat origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of FAS-L of rat origin |
| Entrez Gene | 356 |
|---|
Application
Western blot analysis of FAS-L expression in K-562 (A) and HL-60 (B) whole cell lysates immunoprecipitated with FAS-L (4H9): and detected with FAS-L (N-20): .
Application
Western blot analysis of FAS-L expression in K-562 (A) and HL-60 (B) whole cell lysates immunoprecipitated with FAS-L (NOK-1): and detected with FAS-L (N-20): .
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue at high magnification showing membrane and cytoplasmic localization.| Application Notes | recommended for detection of FAS-L of mouse, rat and human origin by WB, IF, IHC(P) and ELISA also reactive with additional species, including porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
