Application
Western blot analysis of Dok-1 expression in Jurkat (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates.Application
Western blot analysis of Dok-1 expression in K-562 (A) and HEL 92.1.7 (B) whole cell lysates.Application
Western blot analysis of Dok-1 expression in non-transfected: (A) and mouse Dok-1 transfected: (B) 293T whole cell lysates.Application
Antibodies tested include Dok-1 (A-3): (A) and Dok-1 (M-276): (B-C).Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.| Product Name | Dok-1 Antibody (M-276) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-276 mapping at the N-terminus of Dok-1 (GAP-associated p62) of mouse origin |
| Modification Notes | Dok-1 associates with the Ras GTPase-activating protein (Ras GAP) upon tyrosine phosphorylation. Evidence suggests that Dok-1 (also designated p62dok) is a substrate of the constitutive tyrosine kinase activity of p210 Bcr-Abl, a fusion protein caused by the t(9;22) translocation and associated with chronic myelogenous leukemia. Dok-1, as well as the tyrosine kinase substrates IRS-1 and Cas, are members of a class of “docking” proteins which contain multiple tyrosine residues and putative SH2 binding sites. Dok-1 is suspected to be the substrate phosphorylated in response to stimulation by a number of growth factors, including PDGF, VEGF, insulin and IGF. Dok-2 (also designated p56dok) has also been identified as a potential mediator of the effects of p210 Bcr-Abl. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Dok-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-276 mapping at the N-terminus of Dok-1 (GAP-associated p62) of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-276 mapping at the N-terminus of Dok-1 (GAP-associated p62) of mouse origin |
| Entrez Gene | 1796 |
|---|
Application
Western blot analysis of Dok-1 expression in Jurkat (A), K-562 (B) and HEL 92.1.7 (C) whole cell lysates.
Application
Western blot analysis of Dok-1 expression in K-562 (A) and HEL 92.1.7 (B) whole cell lysates.
Application
Western blot analysis of Dok-1 expression in non-transfected: (A) and mouse Dok-1 transfected: (B) 293T whole cell lysates.
Application
Antibodies tested include Dok-1 (A-3): (A) and Dok-1 (M-276): (B-C).
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing cytoplasmic staining.| Application Notes | recommended for detection of Dok-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
