Application
Western blot analysis of FBXO4 expression in non-transfected: (A) and mouse FBXO4 transfected: (B) 293T whole cell lysates.| Product Name | FBXO4 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 59-358 mapping within an internal region of FBXO4 of human origin |
| Modification Notes | F-box proteins are critical components of the SCF (Skp1-CUL-1-F-box protein)-type E3 ubiquitin ligase complex and are involved in substrate recognition and recruitment for ubiquitination. F-box proteins are members of a large family that regulates cell cycle, immune response, signalling cascades and developmental programs by targeting proteins, such as cyclins, cyclin-dependent kinase inhibitors, I˚B-å and ∫-catenin, for degradation by the proteasome after ubiquitination. F-box only protein 4 (FBXO4) is a substrate recognition component of the SCF-type E3 ubiquitin ligase complex, possibly involved in the recognition and binding to phosphorylated target proteins. FBXO4 directly interacts with Skp1 p19 and CUL-1 within the SCF-type E3 complex and has been found to recognize TRF1 and promote its ubiquitination. FBXO4 is expressed as two isoforms produced by alternative splicing. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of FBXO4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 59-358 mapping within an internal region of FBXO4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 59-358 mapping within an internal region of FBXO4 of human origin |
Application
Western blot analysis of FBXO4 expression in non-transfected: (A) and mouse FBXO4 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of FBXO4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
