Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon cancer tissue showing cytoplasmic staining of tumor cells.Application
Western blot analysis of Bcl-xS/L expression in K-562 whole cell lysate.Application
Western blot analysis of Bcl-x expression in non-transfected: (A) and human Bcl-x transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Bcl-xS/L expression in non-transfected control (A) and Bcl-xS/L siRNA transfected (B) HeLa cells. Blot probed with Bcl-xS/L (S-18): . Actin (I-19): used as specificity and loading control.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane and cytoplasmic localization (A). Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic immunostaining and nuclear DAPI counterstain (B).Application
Western blot analysis of Bcl-xL expression in BJAB (A) and Ramos (B) whole cell lysates.| Product Name | Bcl-xS/L Antibody (S-18) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Bcl-xS/L of human origin |
| Modification Notes | The Bcl-2 gene was isolated at the chromosomal breakpoint of t(14;18) bearing follicular B cell lymphomas. Bcl-2 blocks cell death following a variety of stimuli and confers a death-sparing effect to certain hematopoietic cell lines following growth factor withdrawal. A second protein, designated Bcl-associated X protein (Bax) p21, has extensive amino acid homology with Bcl-2 and both homodimerizes and heterodimerizes with Bcl-2. Over-expression of Bax accelerates apoptotic death induced by cytokine deprivation in an IL-3-dependent cell line, and Bax also counters the death repressor activity of Bcl-2. Bcl-x, one of several additional proteins with sequence homology to Bcl-2, is expressed as Bcl-xL, a 233 amino acid protein with 43% sequence identity with Bcl-2 that suppresses cell death, and Bcl-xS, a shorter variant that is 178 amino acids in length and lacks a 63 amino acid region (amino acids 126-188) found in Bcl-xL and which functions as a dominant inhibitor of Bcl-2. A further apoptosis-inducing protein, Bad, dimerizes both with Bcl-xL and to a lesser extent with Bcl-2, thus displacing Bax and inducing apoptosis. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISAChIP |
| recommended for detection of Bcl-xS and Bcl-xL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of Bcl-xS/L of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Bcl-xS/L of human origin |
| Entrez Gene | 598 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human colon cancer tissue showing cytoplasmic staining of tumor cells.
Application
Western blot analysis of Bcl-xS/L expression in K-562 whole cell lysate.
Application
Western blot analysis of Bcl-x expression in non-transfected: (A) and human Bcl-x transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Bcl-xS/L expression in non-transfected control (A) and Bcl-xS/L siRNA transfected (B) HeLa cells. Blot probed with Bcl-xS/L (S-18): . Actin (I-19): used as specificity and loading control.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human colon carcinoma tissue showing membrane and cytoplasmic localization (A). Immunofluorescence staining of methanol-fixed NIH/3T3 cells showing cytoplasmic immunostaining and nuclear DAPI counterstain (B).
Application
Western blot analysis of Bcl-xL expression in BJAB (A) and Ramos (B) whole cell lysates.| Application Notes | recommended for detection of Bcl-xS and Bcl-xL of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
