Application
Western blot analysis of uPAR expression in MCF7 (A), HeLa (B), K-562 (C), LADMAC (D) and SHP-77 (E) whole cell lysates.Application
Western blot analysis of uPAR expression in non-transfected: (A) and human uPAR transfected: (B) 293T whole cell lysates.Application
Western blot analysis of uPAR expression in HeLa whole cell lysate.Application
Western blot analysis of uPAR expression in MCF7 whole cell lysate.| Product Name | uPAR Antibody (FL-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-290 representing full length uPAR (urokinase plasminogen activator surface receptor) of human origin |
| Modification Notes | Urokinase plasminogen activator receptor (uPAR), also designated CD87, is a glycoprotein I-anchored surface receptor specific for urokinase plasminogen activator (uPA). Upon binding to uPAR, uPA converts the surface bound, large serum b-globulin, plasminogen to plasmin. Plasmin, which is also designated fibrinolysin, is a Trypsin- like enzyme that acts on Arg-Lys bonds and induces pericellular proteolysis in fibrin and fibrinogen, and thereby contributes to the systematic activation of the coagulation cascade. This pathway is observed during re-epithelialization of lesions, wound healing and tissue remodeling. uPA and uPAR are known to be overexpressed in mesenchymal and epithelial origin tumor cells and are required for tumor invasion and metastasis. Ras, MEK, ERK and MLCK function as downstream effectors in the uPAR-dependent signaling cascade, which is initiated by uPA binding, and promotes cellular migration in an integrin selective manner. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of uPAR of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-290 representing full length uPAR (urokinase plasminogen activator surface receptor) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-290 representing full length uPAR (urokinase plasminogen activator surface receptor) of human origin |
| Entrez Gene | 5329 |
|---|
Application
Western blot analysis of uPAR expression in MCF7 (A), HeLa (B), K-562 (C), LADMAC (D) and SHP-77 (E) whole cell lysates.
Application
Western blot analysis of uPAR expression in non-transfected: (A) and human uPAR transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of uPAR expression in HeLa whole cell lysate.
Application
Western blot analysis of uPAR expression in MCF7 whole cell lysate.| Application Notes | recommended for detection of uPAR of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
