Application
Western blot analysis of IL-2 expression in IB4 whole cell lysate.Application
Western blot analysis of IL-2 expression in MOLT-4 (A) and HuT 78 (B) whole cell lysates.Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.Application
Western blot analysis of human recombinant IL-2 fusion protein.| Product Name | IL-2 Antibody (H-133) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 21-153 representing mature IL-2 of human origin |
| Modification Notes | Lymphokines are a group of signaling molecules involved in communication between cells of the immune system. Lymphokines secreted by activated lymphocytes include proteins such as IL-2. This protein is secreted primarily by helper T cells that have been activated through the T cell receptor complex or by other mitogens. Cells targeted by IL-2 include activated T helper and cytotoxic T cells, inducing their proliferation. The secretion of IL-2 can also act as a growth factor for B cells. To date, three different IL-2-dependent signal transduction pathways have been identified: the c-Fos/c-Jun induction pathway mediated by Src family protein-tyrosine kinases; the c-Myc induction pathway; and the Rapamycin-sensitive pathway; all of which result in the induction of Bcl-2. In addition, the transcription factor NFAT has been shown to play a major role in the regulation of IL-2 transcription and correlates to an age-related decline in the expression of IL-2. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFIHCELISA |
| recommended for detection of IL-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 21-153 representing mature IL-2 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 21-153 representing mature IL-2 of human origin |
| Entrez Gene | 3558 |
|---|
Application
Western blot analysis of IL-2 expression in IB4 whole cell lysate.
Application
Western blot analysis of IL-2 expression in MOLT-4 (A) and HuT 78 (B) whole cell lysates.
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human skin tissue showing cytoplasmic staining of epidermal cells.
Application
Immunoperoxidase staining of formalin-fixed, paraffin-embedded human epididymis tissue showing cytoplasmic staining of glandular cells at low (A) and high (B) magnification. Kindly provided by The Swedish Human Protein Atlas (HPA) program.
Application
Western blot analysis of human recombinant IL-2 fusion protein.| Application Notes | recommended for detection of IL-2 of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
