Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human CaMKIV transfected HEK 293T cells (B).Application
Western blot analysis of CaMKIV expression in non-transfected: (A) and human CaMKIV transfected: (B) 293 whole cell lysates.Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with CaMKIV (H-146), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.Application
Western blot analysis of CaMKIV expression in Jurkat whole cell lysate.| Product Name | CaMKIV Antibody (H-146) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 328-473 mapping at the C-terminus of CaMKIV of human origin |
| Modification Notes | The Ca2+/calmodulin-dependent protein kinases (CaM kinases) comprise a structurally related subfamily of serine/threonine kinases which include CaMKI, CaMKII and CaMKIV. CaMKII is a ubiquitously expressed serine/threonine protein kinase that is activated by Ca2+and calmodulin (CaM) and has been implicated in regulation of the cell cycle and transcription. There are four CaMKII isozymes designated å, ∫, © and ∂, which may or may not be co-expressed in the same tissue type. CaMKIV is stimulated by Ca2+ and CaM but also requires phosphorylation by a CaMK for full activation. Stimulation of the T cell receptor CD3 signaling complex with an anti-CD3 monoclonal antibody leads to a 10-40 fold increase in CaMKIV activity. An additional kinase, CaMKK, functions to activate CaMKI through the specific phosphorylation of the regulatory Threonine residue at position 177. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of CaMKIV and Calspermin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 328-473 mapping at the C-terminus of CaMKIV of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 328-473 mapping at the C-terminus of CaMKIV of human origin |
| Entrez Gene | 814 |
|---|
Application
Immunofluorescence staining of methanol-fixed untransfected (A) and human CaMKIV transfected HEK 293T cells (B).
Application
Western blot analysis of CaMKIV expression in non-transfected: (A) and human CaMKIV transfected: (B) 293 whole cell lysates.
Application
Indirect, intracellular FCM analysis of fixed and permeabilized Jurkat cells stained with CaMKIV (H-146), followed by FITC-conjugated goat anti-rabbit IgG: . Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Immunofluorescence staining of methanol-fixed Jurkat cells showing nuclear staining.
Application
Western blot analysis of CaMKIV expression in Jurkat whole cell lysate.| Application Notes | recommended for detection of CaMKIV and Calspermin of human and, to a lesser extent, mouse and rat origin by WB, IP, IF, ELISA and indirect FCM using PE (sc-3739)- and FITC (sc-2012)-conjugated goat anti-rabbit IgG: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
