Application
Western blot analysis of PTP expression in non-transfected 293T: (A), human PTP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Product Name | LAR Antibody (H-70) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1081-1150 mapping to an internal region of LAR of human origin |
| Modification Notes | Protein tyrosine phosphatases, or PTPs, are type I transmembrane proteins, membrane associated proteins or proteins localized in nuclei. Examples of transmembrane PTPs are LAR, PTPå, PTP∫, PTP©, PTP∂, PTPe, PTPΩ, PTP˚ and PTPµ. Transmembrane PTPs play diverse roles during development and in adult tissues. Immunodepletion studies have suggested LAR to be a regulator of insulin receptor phosphorylation. PTPa activity is increased twofold in response to phorbol ester stimulation, resulting in serine phosphorylation either directly or indirectly by members of the PKC family. Overexpression of v-H-ras and Neu, but not Myc or Int2, in mammary tumors has been shown to induce PTPe expression . PTPµ localizes to points of cell contact and may be involved in regulating the assembly and disassembly of cadherin/catenin complexes in vivo. PTPµ and PTP˚ share a conserved amino-terminal 160 amino acid MAM domain which facilitates homophilic binding. An alternative splicing event leads to a nervous tissue-specific chondroitin sulfate proteoglycan called phosphacan, which represents the amino terminal portion of PTPΩ. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LAR of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1081-1150 mapping to an internal region of LAR of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1081-1150 mapping to an internal region of LAR of human origin |
| Entrez Gene | 5792 |
|---|
Application
Western blot analysis of PTP expression in non-transfected 293T: (A), human PTP transfected 293T: (B) and NIH/3T3 (C) whole cell lysates.| Application Notes | recommended for detection of LAR of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
