Application
Western blot analysis of Lyn expression in A-431 whole cell lysate.Application
Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .Application
Western blot analysis of Lyn expression in non-transfected 293T: (A), mouse Lyn transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Immunofluorescence staining of normal mouse lymph node frozen section showing membrane and cytoplasmic staining.Application
Western blot analysis of Lyn expression in BJAB (A), HL-60 (B) and 3611-RF (C) whole cell lysates.Application
Western blot analysis of Lyn expression in HL-60 (A), MCF7 (B), SK-BR-3 (C), 3611-RF (D) and BJAB (E) whole cell lysates.| Product Name | Lyn Antibody (44) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping at the N-terminus of Lyn of human origin |
| Modification Notes | Src is the human homolog of the v-src gene of the Rous sarcoma virus, also known as avian sarcoma virus or ASV. Src is the first proto-oncogenic non-receptor tyrosine kinase characterized in human. By virtue of common structural motifs, the Src family is composed of nine members in vertebrates, including Src, Yes, Fgr, Frk, Fyn, Lyn, Hck, Lck and Blk. Src-family kinases transduce signals that are involved in the control of a variety of cellular processes, including proliferation, differentiation, motility, and adhesion. Src-family kinases contain an amino terminal cell membrane anchor followed by an SH3 domain and an SH2 domain involved in modular association and activation, respectively. Src-family kinases are normally maintained in an inactive state and can be activated transiently during cellular events such as mitosis. The human Lyn gene maps to chromosome 8q12 and encodes a 505 amino acid protein. Hematopoietic tissues predominantly express Lyn, which influences normal immunoglobulin production and regulation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFFCELISA |
| recommended for detection of Lyn p56/p53 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping at the N-terminus of Lyn of human origin |
|---|---|
| Tissue Specificity | epitope mapping at the N-terminus of Lyn of human origin |
| Entrez Gene | 4067 |
|---|
Application
Western blot analysis of Lyn expression in A-431 whole cell lysate.
Application
Intracellular FCM analysis of fixed and permeabilized BJAB cells. Black line histogram represents the isotype control, normal rabbit IgG: .
Application
Western blot analysis of Lyn expression in non-transfected 293T: (A), mouse Lyn transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Immunofluorescence staining of normal mouse lymph node frozen section showing membrane and cytoplasmic staining.
Application
Western blot analysis of Lyn expression in BJAB (A), HL-60 (B) and 3611-RF (C) whole cell lysates.
Application
Western blot analysis of Lyn expression in HL-60 (A), MCF7 (B), SK-BR-3 (C), 3611-RF (D) and BJAB (E) whole cell lysates.| Application Notes | recommended for detection of Lyn p56/p53 of mouse, rat and human origin by WB, IP, IF, FCM and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
