Application
Western blot analysis of Pax-6 expression in non-transfected: (A) and human Pax-6 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of Pax-6 expression in non-transfected: (A) and mouse Pax-6 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed Y79 cells showing nuclear localization.Application
Western blot analysis of Pax-6 expression in Y79 nuclear extract (A) and rat eye tissue extract (B).| Product Name | Pax-6 Antibody (H-295) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 264-422 mapping at the C-terminus of Pax-6 of human origin |
| Modification Notes | Pax genes contain paired domains with strong homology to genes in Drosophila which are involved in programming early development. Lesions in the Pax-6 gene account for most cases of aniridia, a congenital malformation of the eye, chiefly characterized by iris hypoplasia, which can cause blindness. Pax-6 is involved in other anterior segment malformations besides aniridia, such as Peters anomaly, a major error in the embryonic development of the eye with corneal clouding with variable iridolenticulocorneal adhesions. The Pax-6 gene encodes a transcriptional regulator that recognizes target genes through its paired-type DNA-binding domain. The paired domain is composed of two distinct DNA-binding subdomains, the amino-terminal subdomain and the carboxy-terminal subdomain, which bind respective consensus DNA sequences. The human Pax-6 gene produces two alternatively spliced isoforms that have the distinct structure of the paired domain. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of Pax-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 264-422 mapping at the C-terminus of Pax-6 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 264-422 mapping at the C-terminus of Pax-6 of human origin |
| Entrez Gene | 5080 |
|---|
Application
Western blot analysis of Pax-6 expression in non-transfected: (A) and human Pax-6 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of Pax-6 expression in non-transfected: (A) and mouse Pax-6 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed Y79 cells showing nuclear localization.
Application
Western blot analysis of Pax-6 expression in Y79 nuclear extract (A) and rat eye tissue extract (B).| Application Notes | recommended for detection of Pax-6 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
