Application
Western blot analysis of Ran BP-3 expression in Jurkat (A) and Y79 (B) nuclear extracts.| Product Name | Ran BP-3 Antibody (M-175) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-175 mapping at the N-terminus of Ran BP-3 of mouse origin |
| Modification Notes | The small Ras-related protein Ran, also called TC4, is a nuclear localized GTPase implicated in a diverse array of cellular processes including DNA replication, entry into and exit from mitosis and the transport of RNA and proteins through the nuclear pore complex. Like Ras, active Ran GTP and inactive Ran GDP levels are tightly regulated by guanine nucleotide exchange factors (GEFs) and GTPase activating proteins (GAPs). The abundant GEF, RCC1 (regulator of chromosome condensation 1), increases the rate at which Ran exchanges GDP for GTP. Ran BP-3 acts as a scaffold protein to promote the efficient assembly of export complexes. Specifically, Ran BP-3 promotes binding of Crm1 to RCC1 in the presence of Ran. Ran BP-3 has also been shown to bind ∫-catenin, thereby inhibiting the Wnt signaling pathway. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Ran BP-3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-175 mapping at the N-terminus of Ran BP-3 of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-175 mapping at the N-terminus of Ran BP-3 of mouse origin |
Application
Western blot analysis of Ran BP-3 expression in Jurkat (A) and Y79 (B) nuclear extracts.| Application Notes | recommended for detection of Ran BP-3 of mouse, rat and, to a lesser extent, human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
