Application
Western blot analysis of JRAB expression in non-transfected: (A) and human JRAB transfected: (B) 293T whole cell lysates.| Product Name | JRAB Antibody (M-84) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-84 mapping at the N-terminus of JRAB of mouse origin |
| Modification Notes | JRAB (junctional Rab13-binding protein, MICAL-like protein 2) is a 904 amino acid protein with one CH (calponin-homology) domain and one LIM zinc-binding domain. JRAB has been shown to interact with Rab13 and Rab8 to facilitate cellular transport of claudin-1, occludin, and E-cadherin. This interaction is vital for the coordination of the assembly of tight junctions (TJs) and adherens junctions (AJs). Dynamic turnover (endocytic recycling) of cell-to-cell AJs and TJs is essential for epithelial morphogenesis during normal development and differentiation. The endocytic recycling of occludin and cluadin proteins is part of an ongoing process of restructuring and maintaining cell junctions, especially at TJs. JRAb and Rab13 have also been implicated in the carcinoma metastasis event of epithelial cell scattering. This event shows Rab13 and JRAB colocalizing with F-Actin in lamellipodial structures prior to cell scattering. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of JRAB of mouse and rat origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-84 mapping at the N-terminus of JRAB of mouse origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-84 mapping at the N-terminus of JRAB of mouse origin |
Application
Western blot analysis of JRAB expression in non-transfected: (A) and human JRAB transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of JRAB of mouse and rat origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
