Application
Western blot analysis of ERCC1 expression in non-transfected 293T: (A), mouse ERCC1 transfected 293T: (B) and SK-BR-3 (C) whole cell lysates.Application
Western blot analysis of ERCC1 expression in non-transfected: (A) and human ERCC1 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of ERCC1 expression in A-431 (A) and HeLa + UV (B) whole cell lysates.| Product Name | ERCC1 Antibody (FL-297) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-297 representing full length DNA excision repair protein 1 (ERCC1) of human origin |
| Modification Notes | Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer; it is commonly due to deficiencies in DNA repair enzymes. The most frequent mutations are found in the XP genes from group A through G and group V, which encode for nucleotide excision repair proteins. XPF, which is also designated ERCC4 or ERCC11, is a protein that associates directly with the excision repair cross-complementing 1 (ERCC1) factor. ERCC-1, a functional homolog of Rad10 in S. cerevisiae, is a component of a structure-specific endonuclease that is responsible for 5’ incisions during DNA repair. The ERCC1-XPF endo-nuclease preferentially cleaves one strand of DNA between duplex and single-stranded regions near borders of the stem-loop structure and, thereby, contributes to the initial steps of the nucleotide excision repair process. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAGSChIP |
| recommended for detection of ERCC1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-297 representing full length DNA excision repair protein 1 (ERCC1) of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-297 representing full length DNA excision repair protein 1 (ERCC1) of human origin |
| Entrez Gene | 2067 |
|---|
Application
Western blot analysis of ERCC1 expression in non-transfected 293T: (A), mouse ERCC1 transfected 293T: (B) and SK-BR-3 (C) whole cell lysates.
Application
Western blot analysis of ERCC1 expression in non-transfected: (A) and human ERCC1 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of ERCC1 expression in A-431 (A) and HeLa + UV (B) whole cell lysates.| Application Notes | recommended for detection of ERCC1 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
