Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of POL H expression in HeLa whole cell lysate.| Product Name | POL H Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 414-713 of POL H of human origin |
| Modification Notes | Xeroderma pigmentosum (XP) is an autosomal recessive disorder characterized by a genetic predisposition to sunlight-induced skin cancer due to deficiencies in the DNA repair enzymes. The most frequent mutations are found in the XP genes of group A through G and group V, which encode nucleotide excision repair proteins. The XPA gene encodes a zinc metalloprotein that preferentially binds to DNA damaged by UV radiation and chemical carcinogens and is required for the incision step during nucleotide excision repair The XPB and XPD genes encode DNA helicases involved in several DNA metabolic pathways, including DNA repair and transcription, and the XPG gene product is an endonuclease that cuts on the 3' side of a DNA lesion during nucleotide excision repair. Molecular defects in the XP variant (POL H) group maintain normal excision repair, yet they result in a substantial reduction in the ability to synthesize intact daughter DNA strands during DNA replication following DNA damage . |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of POL H of human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 414-713 of POL H of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 414-713 of POL H of human origin |
| Entrez Gene | 5429 |
|---|
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of POL H expression in HeLa whole cell lysate.| Application Notes | recommended for detection of POL H of human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
