Application
Antibodies tested include Pol II (C-21): and TFIID (TBP) (SI-1): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- or WT thymocytes. Data kindly provided by J. Imbert and reproduced from Yeh, J.H., et al. 2002. Nucleic Acids Res. 30: 1944-1951, with permission from Oxford University Press.Application
Antibodies tested include Pol II (C-21): (A-C) and Pol II (N-20): (D-F).| Product Name | Pol II Antibody (C-21) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse origin |
| Modification Notes | RNA polymerase II (Pol II) is an enzyme that is composed of twelve subunits and is responsible for the transcription of protein-coding genes. Transcription initiation requires Pol II-mediated recruitment of transcription machinery to a target promoter, thereby allowing transcription to begin. The largest subunit of Pol II (referred to as RPB1 or RPB205) is a 1,840 amino acid protein that contains one C2H2-type zinc finger and a C-terminal domain comprised of several heptapeptide repeats. Although Pol II function requires the cooperation of all twelve subunits, the largest subunit conveys Pol II catalytic activity and, together with the second largest subunit, forms the active center of the Pol II enzyme. Additionally, the large subunit participates in forming the DNA-binding domain of Pol II, a groove that is necessary for transcription of the DNA template. Without proper function of the large subunit, mRNA synthesis and subsequent transcription elongation cannot occur. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISAChIP |
| recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with NF-1 protein also reactive with additional species, including canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse origin |
|---|---|
| Tissue Specificity | epitope mapping within the tandem repeat domain of the large subunit of RNA polymerase II (RPB1) of mouse origin |
| Entrez Gene | 5430 |
|---|
Application
Antibodies tested include Pol II (C-21): and TFIID (TBP) (SI-1): . CD3ε and Oct-2 promoter regions were employed as positive and negative controls, respectively. DNA was isolated from Rag-/- or WT thymocytes. Data kindly provided by J. Imbert and reproduced from Yeh, J.H., et al. 2002. Nucleic Acids Res. 30: 1944-1951, with permission from Oxford University Press.
Application
Antibodies tested include Pol II (C-21): (A-C) and Pol II (N-20): (D-F).| Application Notes | recommended for detection of Pol II of mouse, rat and human origin by WB, IP, IF and ELISA may cross-react with NF-1 protein also reactive with additional species, including canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
