Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.Application
Western blot analysis of RFC4 expression in non-transfected 293T: (A), human RFC4 transfected 293T: (B) and HeLa (C) whole cell lysates.Application
Western blot analysis of RFC4 expression in non-transfected: (A) and human RFC4 transfected: (B) 293T whole cell lysates.Application
Western blot analysis of RFC4 expression in non-transfected: (A) and mouse RFC4 transfected: (B) 293T whole cell lysates.Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of RFC4 expression in HeLa (A) and LNCaP (B) whole cell lysates.| Product Name | RFC4 Antibody (H-183) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 181-363 of RFC4 of human origin |
| Modification Notes | Replication factor C (RFC) is an essential DNA polymerase accessory protein that is required for numerous aspects of DNA metabolism including DNA replication, DNA repair, and telomere metabolism. RFC is a heteropentameric complex that recognizes a primer on a template DNA, binds to a primer terminus, and loads proliferating cell nuclear antigen (PCNA) onto DNA at primer-template junctions in an ATP-dependent reaction. All five of the RFC subunits share a set of related sequences (RFC boxes) that include nucleotide-binding consensus sequences. Four of the five RFC genes (RFC1, RFC2, RFC3, and RFC4) have consensus ATP-binding motifs. The small RFC proteins, RFC2, RFC3, RFC4 and RFC5, interact with Rad24, whereas the RFC1 subunit does not. Specifically, RFC4 plays a role in checkpoint regulation. RFC4 is a component of BASC (for BRCA1-associated genome surveillance complex) which serves as a sensor for abnormal DNA structures and/or as a regulator of the postreplication repair process. The human RFC4 gene maps to chromosome 3q27.3 and encodes the RFC4 subunit. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of RFC4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 181-363 of RFC4 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 181-363 of RFC4 of human origin |
| Entrez Gene | 5984 |
|---|
Application
Immunofluorescence staining of formalin-fixed Hep G2 cells showing nuclear localization.
Application
Western blot analysis of RFC4 expression in non-transfected 293T: (A), human RFC4 transfected 293T: (B) and HeLa (C) whole cell lysates.
Application
Western blot analysis of RFC4 expression in non-transfected: (A) and human RFC4 transfected: (B) 293T whole cell lysates.
Application
Western blot analysis of RFC4 expression in non-transfected: (A) and mouse RFC4 transfected: (B) 293T whole cell lysates.
Application
Immunofluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of RFC4 expression in HeLa (A) and LNCaP (B) whole cell lysates.| Application Notes | recommended for detection of RFC4 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
