Application
Western blot analysis of MCM5 expression in KNRK (A), HL-60 (B), HeLa (C) and A-431 (D) nuclear extracts.Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear localization.Application
Western blot analysis of MCM5 expression in non-transfected control (A) and MCM5 siRNA transfected (B) HeLa cells. Blot probed with MCM5 (H-300): . α-actinin (H-2): used as specificity and loading control.Application
Western blot analysis of MCM5 expression in HL-60 nuclear extract.| Product Name | MCM5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MCM5 of human origin |
| Modification Notes | The mini-chromosome maintenance (MCM) family of proteins, including MCM2, MCM3, MCM4 (Cdc21), MCM5 (Cdc46), MCM6 (Mis5) and MCM7 (Cdc47), are regulators of DNA replication that act to ensure replication occurs only once in the cell cycle. Expression of MCM proteins increases during cell growth, peaking at G1 to S phase. The MCM proteins each contain an ATP-binding motif, which is predicted to mediate ATP-dependent opening of double-stranded DNA. MCM proteins are regulated by E2F transcription factors, which induce MCM expression, and by protein kinases, which interact with MCM proteins to maintain the postreplicative state of the cell. MCM2/MCM4 complexes function as substrates for Cdc2/cyclin B in vitro. Cleavage of MCM3, which can be prevented by caspase inhibitors, results in the inactivation during apoptosis of the MCM complex, which is composed of, at least, MCM2–6. A complex composed of MCM4, MCM6 and MCM7 has been shown to be involved in DNA helicase activity, and MCM5 is involved in IFN-©-induced Stat1å transcription activation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of MCM5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MCM5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of MCM5 of human origin |
| Entrez Gene | 4174 |
|---|
Application
Western blot analysis of MCM5 expression in KNRK (A), HL-60 (B), HeLa (C) and A-431 (D) nuclear extracts.
Application
Immuno?fluorescence staining of methanol-fixed HeLa cells showing nuclear localization.
Application
Western blot analysis of MCM5 expression in non-transfected control (A) and MCM5 siRNA transfected (B) HeLa cells. Blot probed with MCM5 (H-300): . α-actinin (H-2): used as specificity and loading control.
Application
Western blot analysis of MCM5 expression in HL-60 nuclear extract.| Application Notes | recommended for detection of MCM5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
