Application
Western blot analysis of ASC-1 expression in non-transfected: (A) and mouse ASC-1 transfected: (B) 293T whole cell lysates.| Product Name | ASC-1 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 282-581 mapping at the C-terminus of ASC-1 of human origin |
| Modification Notes | Activating signal co-integrator-1 (ASC-1), originally identified as TRIP4, is a transcriptional co-activator of nuclear receptors that associates with specific components of the RNA polymerase II complex and binds the basal transcription factors TBP and TFIIA. ASC-1 functions with the transcription integrators SRC-1 and CBP/p300 through its zinc finger motif and is dependent on their ligand-dependent transactivation domain, AF2. Endogenous ASC-1 in HeLa cells is predominantly a nuclear protein. Under conditions of serum starvation, ASC-1 localizes to the cytoplasm. However, when serum starved in the presence of ligand of coexpressed CBP or SRC-1, ASC-1 remains in the nucleus. This behavior of ASC-1 suggests that it may play an important role in establishing distinct co-activator complexes under different cellular conditions. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ASC-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 282-581 mapping at the C-terminus of ASC-1 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 282-581 mapping at the C-terminus of ASC-1 of human origin |
| Entrez Gene | 9325 |
|---|
Application
Western blot analysis of ASC-1 expression in non-transfected: (A) and mouse ASC-1 transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ASC-1 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine and porcine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
