Application
Western blot analysis of API5 expression in HeLa (A), KNRK (B), A549 (C), SH-SY5Y (D) and A-431 (E) nuclear extracts.Application
Western blot analysis of API5 expression in non-transfected: (A) and human API5 transfected: (B) 293T whole cell lysates and Y79 (C) and WI 38 (D) nuclear extracts.| Product Name | API5 Antibody (H-300) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-300 mapping at the N-terminus of API5 of human origin |
| Modification Notes | API5 (apoptosis inhibitor 5), also known as AAC11 (antiapoptosis clone 11 protein), FIF (fibroblast growth factor 2-interacting factor), MIG8, XAGL or API5L1, is a widely expressed antiapoptotic nuclear protein that is highly conserved from flies to humans. API5 contains a nuclear localization sequence, an LxxLL motif, a leucine zipper domain and a transactivation domain flanked by two acidic domains. API5 forms a nuclear localized complex with FGF-2 and may mediate FGF-2-dependent signaling. API5 is believed to function as a transcription regulator and is able to regulate the synthesis of MMP-2 (matrix metalloproteinase-2). In addition, API5 is known to specifically suppress E2F-dependent apoptosis. It is expressed in a variety of cancer cell lines and its expression is linked to tumor progression and the degree of malignancy. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of API5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-300 mapping at the N-terminus of API5 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-300 mapping at the N-terminus of API5 of human origin |
Application
Western blot analysis of API5 expression in HeLa (A), KNRK (B), A549 (C), SH-SY5Y (D) and A-431 (E) nuclear extracts.
Application
Western blot analysis of API5 expression in non-transfected: (A) and human API5 transfected: (B) 293T whole cell lysates and Y79 (C) and WI 38 (D) nuclear extracts.| Application Notes | recommended for detection of API5 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
