Application
Western blot analysis of ALF expression in Jurkat nuclear extract.| Product Name | ALF Antibody (H-57) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 18-74 mapping near the N-terminus of ALF of human origin |
| Modification Notes | ALF (TFIIA å/∫ like factor), also known as GTF2A1L (general transcription factor IIA 1-like), is a nuclear and cytoplasmic protein that is specifically expressed in haploid spermatids in the testis. ALF is believed to play a role in spermatogenesis, functioning as a transcription factor, and is related to the large a/b subunit of TFIIA, existing as the germ cell-specific paralog. Similar to TFIIA-å/∫, ALF associates with the smaller TFIIA subunit, TFIIA-©, and functions to stabilize the interaction between TBP and DNA by binding directly to TBP and the DNA (at the TATA box), thus forming a TBP/ALF/TATA complex which mediates the transcriptional output of a gene. Low expression levels of ALF have been associated with abnormal spermatogenesis and male infertility, further implying a role for ALF in spermatogenic cell function. The transcriptional expression of ALF appears to be regulated by DNA methylation. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ALF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 18-74 mapping near the N-terminus of ALF of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 18-74 mapping near the N-terminus of ALF of human origin |
| Entrez Gene | 11036 |
|---|
Application
Western blot analysis of ALF expression in Jurkat nuclear extract.| Application Notes | recommended for detection of ALF of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine and bovine: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
