Application
Western blot analysis of PI 3-kinase p85α phosphorylation expression in untreated (A) and sorbitol treated (B) 293 whole cell lysates.| Product Name | p-PI 3-kinase p85α Antibody (Tyr 467) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | recommended for detection of Tyr 508 phosphorylated PI 3-kinase p85å of m, r and h origin by WB, IP and IF |
| Modification | Phosphoralated |
| Modification Notes | Phosphatidylinositol 3-kinase (PI 3-kinase) phosphorylates the 3' OH position of the inositol ring of inositol lipids and is composed of p85 and p110 subunits. PI 3-kinase p85 lacks PI 3-kinase activity and acts as an adapter, coupling p110 to activated protein tyrosine kinase. Two forms of p85 have been described (p85å and p85∫), each possessing one SH3 and two SH2 domains. PI 3-kinase p85α, also known as GRB1, phosphatidylinositol 3-kinase regulatory 1 or p85, is a 724 amino acid protein that exists as four alternatively spliced isoforms. Involved in insulin metabolism, defects in the PI 3-kinase p85α gene have been linked to insulin resistance. PI 3-kinase p85α is polyubiquitinated in T-cells by Cbl-b, and has multiple phosphorylated amino acid residues, including a phosphorylated tyrosine residue at position 467. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIF |
| See 1 citations for p-PI 3-kinase p85αAntibody (Tyr 467): |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | recommended for detection of Tyr 508 phosphorylated PI 3-kinase p85å of m r and h origin by WB IP and IF |
|---|---|
| Tissue Specificity | recommended for detection of Tyr 508 phosphorylated PI 3-kinase p85å of m, r and h origin by WB, IP and IF |
| Entrez Gene | 5295 |
|---|
Application
Western blot analysis of PI 3-kinase p85α phosphorylation expression in untreated (A) and sorbitol treated (B) 293 whole cell lysates.| Application Notes | See 1 citations for p-PI 3-kinase p85αAntibody (Tyr 467): |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
