Application
Western blot analysis of TFG expression in non-transfected: (A) and mouse TFG transfected: (B) 293T whole cell lysates.| Product Name | TRK-T3 Antibody (H-210) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 1-210 mapping at the N-terminus of TRK-T3 of human origin |
| Modification Notes | Oncogenic rearrangements of the NTRK1 gene, which encodes the Trk A protein, are frequently detected in thyroid carcinomas. Such rearrangements fuse the NTRK1 tyrosine kinase domain to 5'-end sequences of different genes. TRK-T3 contains 1,412 nucleotides of NTRK1 preceded by 598 nucleotides belonging to TFG (TRK-fused gene), a ubiquitously expressed gene located on chromosome 3. The TRK-T3 protein within the TFG region contains a coiled-coil motif that gives the oncoprotein the capability to form complexes. The cytoplasmic TRK-T3 protein binds to and phosphorylates the Shc and SNT1/FRS2 adaptor proteins, both of which are involved in coupling the receptor tyrosine kinase to the mitogen-activated protein kinase pathway by recruiting Grb2/SOS. SHP-1 also interacts with and down-regulates TRK-T3. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of TRK-T3 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 1-210 mapping at the N-terminus of TRK-T3 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 1-210 mapping at the N-terminus of TRK-T3 of human origin |
| Entrez Gene | 10342 |
|---|
Application
Western blot analysis of TFG expression in non-transfected: (A) and mouse TFG transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of TRK-T3 of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
