Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.| Product Name | Alix Antibody (H-270) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 463-732 mapping near the C-terminus of Alix of human origin |
| Modification Notes | ALG-2-interacting protein (Alix), also designated programmed cell death 6-interacting protein (PDCD6-interacting protein), is a cytoplasmic protein. Alix interacts with apoptosis-associated proteins (ALG-2 and PDCD6) and with the endocytosis-regulator CIN85. Additionally, Alix interacts with the endosomal sorting complexes required for transport (ESCRT) proteins (Tsg101 and CHMP4) and can associate with HIV-1. The endophilins (SH3P4, SH3P8 and SH3P13), enzymes that change curvature of the membrane that are required for early and late steps of coated vesicle formation, also bind to Alix. Alix is involved in the concentration and sorting of cargo proteins of the multivesicular body for incorpoation into vesicles. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of Alix of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 463-732 mapping near the C-terminus of Alix of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 463-732 mapping near the C-terminus of Alix of human origin |
| Entrez Gene | 10015 |
|---|
Application
Immunoperoxidase staining of formalin fixed, paraffin-embedded human gall bladder tissue showing cytoplasmic and membrane staining of glandular cells.| Application Notes | recommended for detection of Alix of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
