Application
Western blot analysis of EF-Ts expression in non-transfected 293T: (A), mouse EF-Ts transfected 293T: (B) and HeLa (C) whole cell lysates.| Product Name | EF-Ts Antibody (H-290) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 36-325 mapping at the C-terminus of EF-Ts of human origin |
| Modification Notes | EF-Ts, also known as TSFM (Ts translation elongation factor, mitochondrial) or COXPD3, is a 325 amino acid protein that is one of 13 mitochondrial-encoded proteins that work together during the elongation phase of protein biosynthesis on the ribosome. Expressed ubiquitously with highest levels present in liver, kidney and skeletal muscle, EF-Ts associates with EF-Tu, a multidomain GTPase with essential functions in translation, and, via this interaction, facilitates the exchange of GDP for GTP, thereby inducing protein elongation. Mutations in the gene encoding EF-Ts are the cause of combined oxidative phosphorylation deficiency type 3 (COXPD3), a condition characterized by defects in the mitochondrial oxidative phosphorylation system and often characterized by severe metabolic acidosis with encephalomyopathy or with hypertrophic cardiomyopathy. Multiple isoforms of EF-Ts exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of EF-Ts of mouse, rat and human origin by WB, IP, IF and ELISA: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 36-325 mapping at the C-terminus of EF-Ts of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 36-325 mapping at the C-terminus of EF-Ts of human origin |
| Entrez Gene | 10102 |
|---|
Application
Western blot analysis of EF-Ts expression in non-transfected 293T: (A), mouse EF-Ts transfected 293T: (B) and HeLa (C) whole cell lysates.| Application Notes | recommended for detection of EF-Ts of mouse, rat and human origin by WB, IP, IF and ELISA: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
