Application
Western blot analysis of ACADS expression in non-transfected 293T: (A), human ACADS transfected 293T: (B) and Hep G2 (C) whole cell lysates and mouse kidney tissue extract (D).Application
Western blot analysis of ACADS expression in non-transfected 293T: (A), mouse ACADS transfected 293T: (B) and Hep G2 (C) whole cell lysates and mouse kidney tissue extract (D).Application
Western blot analysis of ACADS expression in non-transfected: (A) and mouse ACADS transfected: (B) 293T whole cell lysates.| Product Name | ACADS Antibody (H-141) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 104-244 mapping within an internal region of ACADS of human origin |
| Modification Notes | ACADS (acyl-Coenzyme A dehydrogenase, C-2 to C-3 short chain), also known as SCAD or ACAD3, is a 412 amino acid homotetrameric mitochondrial flavoprotein that belongs to the acyl-CoA dehydrogenase family. ACADS catalyzes the rate-limiting step of the mitochondrial fatty acid beta-oxidation pathway. Mutations of ACADS have been associated with fatty acid oxidation defects and metabolic diseases such as short-chain acyl-CoA dehydrogenase deficiency (SCAD deficiency), an autosomal recessive disorder resulting in acute acidosis and muscle weakness in infants and lipid-storage myopathy in adults. SCADS leads to the accumulation of butyrylcarnitine and ethylmalonic acid in blood and urine. ACADS contains four FAD domains. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of ACADS of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other ACAD family members also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 104-244 mapping within an internal region of ACADS of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 104-244 mapping within an internal region of ACADS of human origin |
| Entrez Gene | 35 |
|---|
Application
Western blot analysis of ACADS expression in non-transfected 293T: (A), human ACADS transfected 293T: (B) and Hep G2 (C) whole cell lysates and mouse kidney tissue extract (D).
Application
Western blot analysis of ACADS expression in non-transfected 293T: (A), mouse ACADS transfected 293T: (B) and Hep G2 (C) whole cell lysates and mouse kidney tissue extract (D).
Application
Western blot analysis of ACADS expression in non-transfected: (A) and mouse ACADS transfected: (B) 293T whole cell lysates.| Application Notes | recommended for detection of ACADS of mouse, rat and human origin by WB, IP, IF and ELISA non cross-reactive with other ACAD family members also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
