Application
Western blot analysis of DIS3 expression in non-transfected: (A) and human DIS3 transfected: (B) 293T whole cell lysates and SW480 nuclear extract (C).| Product Name | DIS3 Antibody (T-20) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope mapping within an internal region of DIS3 of human origin |
| Modification Notes | The exosome is a multi-protein complex composed of several highly conserved subunits, some of which are 3’ to 5’ exoribonucleases. The complex is involved in a variety of cellular processes and is responsible for degrading unstable mRNAs that contain AU-rich elements (AREs) in their untranslated 3’ regions. DIS3, also known as RRP44, is a 958 amino acid protein that localizes to both the cytoplasm and the nucleus and contains one PINc domain. Widely expressed with highest expression in testis, DIS3 functions as a component of the exosome exoribonuclease complex and is required for processing of 7S pre-RNA into a mature nuclear complex and, ultimately, for proper mitotic progression. Abnormal expression levels of DIS3 may be associated with colon cancer, suggesting a role for DIS3 in tumorigenesis. Multiple isoforms of DIS3 exist due to alternative splicing events. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of DIS3 of h and r origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope mapping within an internal region of DIS3 of human origin |
|---|---|
| Tissue Specificity | epitope mapping within an internal region of DIS3 of human origin |
| Entrez Gene | 22894 |
|---|
Application
Western blot analysis of DIS3 expression in non-transfected: (A) and human DIS3 transfected: (B) 293T whole cell lysates and SW480 nuclear extract (C).| Application Notes | recommended for detection of DIS3 of h and r origin by WB, IP, IF and ELISA also reactive with additional species, including equine, canine, bovine, porcine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
