Application
Western blot analysis of LIN-41 expression in A-10 whole cell lysate.| Product Name | LIN-41 Antibody (H-269) |
|---|---|
| Antibody Type | Primary Antibodies |
| Antigen Alias | epitope corresponding to amino acids 264-532 mapping within an internal region of LIN-41 of human origin |
| Modification Notes | LIN-41, also called tripartite motif-containing 71 (TRIM71), which was first identified in Caenorhabditis elegans, is responsible for the timing of cell fate determination. By encoding microRNAs (miRNAs), the heterochronic genes let-7 and lin-4 downregulate the gene encoding LIN-41. The miRNAs bind to six complementary sites on the 3' untranslated region (UTR) of the LIN-41 gene. This downregulation positively regulates the timing of the expression of LIN-29, an adult specification transcription factor. Null mutations in the gene encoding LIN-41 lead to the premature development of adult tissues during larval stages. Although LIN-41 is expressed in many different embryonic cell types, it is most highly expressed in the developing limb buds, tail buds and brachial arches. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WBIPIFELISA |
| recommended for detection of LIN-41 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Alternative Names | epitope corresponding to amino acids 264-532 mapping within an internal region of LIN-41 of human origin |
|---|---|
| Tissue Specificity | epitope corresponding to amino acids 264-532 mapping within an internal region of LIN-41 of human origin |
| Entrez Gene | 131405 |
|---|
Application
Western blot analysis of LIN-41 expression in A-10 whole cell lysate.| Application Notes | recommended for detection of LIN-41 of mouse, rat and human origin by WB, IP, IF and ELISA also reactive with additional species, including canine, bovine and avian: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | For short-term storage, store at 4° C. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
